F + , Hfr, and F' strains of Salmonella typhimurium and Salmonella abony.
نویسندگان
چکیده
INTRODUCTION .............................................................. 609 MATERIALS AND METHODS ...................... ........................... 609 Media..................................................................... 609 Strains .................................................................... 609 S. typhimurium ............................................................. 609 S. abony .................................................................... 612 Mating Methods ............................................................... 612 1. Replica plating ........................................................... 613 2. Plate mating ............................................................. 613 3. Broth mating ............................................................. 613 4. Interrupted conjugation .............. .................................... 613 Isolation of Hfr Strains ......... ............... .............................. 613 Isolation of F' Strains .......................... ............................... 613 1. Selection for early entry of a distal gene ........ ......................... 613 2. Selection for recombinants for proximal or distal genes ...... ............ 613 3. Selection for "transmissible Hfr property".... ..... ....................... 613 Transmission of the F Factor ...................... ........................... 613 Testing for the Presence of a '4ansmissible F Factor ........ .. ............... 613 Storage Methods .............................................................. 614 1. Stab cultures ............................................................. 614 2. Slant cultures ........................................................... 614 3. Frozen cultures............................................................ 614 4. Lyophilized cultures .................. .................................... 614 Methods of Testing Phage Sensitivity .................. ....................... 614 Nomenclature ................................................................. 614 RESULTS .................................................................... 614 Mating Methods ............................................................... 614 Stability of Hfr Strains During Storage ................ ...................... 617 Sources of the F+ Strains ........................ ............................. 618 Hfr Strains of S. typhimurium ............ ................................... 618 SR305 (HfrA) ............................................................... 618 SR315 (leu-256/FS21) ............................................ 618 SU418 (HfrB2) .............................................................. 618 SU436 (HfrB3) .............................................................. 620 SA458 (HfrKl-l), SA464 (HfrKl-2), SA537 (HfrKI-3), SA538 (HfrKl-4), SA539 (HfrKl-5), SA639 (HfrKl-6), SA642 (HfrKl-7), and SA967 (HfrKl-8) .............................................................. 620 SA486 (EHfrK3).............................................................. 623 SA534 (HfrK4).............................................................. 623 SA535 (HfrK5)............................................................. 624 SA536 (HfrK6), SA949 (HfrK7), and SA977 (HEfrK8) ...... .................. 624 SA540 (HfrK2-1), SA640 (HEfrK2-2), and SA653 (HfrK2-3) ...... ............ 624 SA654 (HfrK9).............................................................. 624 SA722 (HEfrKO) ............................................................. 624 SA828 (HfrK25) ............................................................. 624 SA949 (HfrK7).............................................................. 624 SA949 (HfrKl4), SA967 (HErK-8), and SA975 (HfrK13) ...... .............. 624 SA955 (HfrK20) and SA965 (HfrKl7) ......... .............................. 626 SA962 (HfrK16).626 SA965 (HfrK7) SA966 (HfrKl9) ............................................................. 626 SA967 (HfrKI-8) ........................................................... 628 SA969 (HfrK5) ............................................................. 628
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ورودعنوان ژورنال:
- Bacteriological reviews
دوره 36 4 شماره
صفحات -
تاریخ انتشار 1972